![]() ![]() ![]() Praesto Q90 demonstrates selectivity equal to Q Sepharose Fast Flow and Capto Q. Results indicate that DMA can be used as a small volume, complementary technique for the mechanical characterisation of chromatography media. Praesto SP (cation) and Praesto Q (anion) chromatography resins are. We found that by using a commercially available Q XL resin in a flow-through mode, endotoxin could be effectively removed from these proteins while. To ascertain whether polymer structure is indicative of mechanical strength, scanning electron microscopy images were also used to study the structural properties of each resin. It is composed of a rigid, high-flow agarose matrix modified with dextran surface extenders and a strong quaternary ammonium (Q) anion exchanger. available S type resins for capture and intermediate purification. The most mechanically robust resin (Capto Q) had a critical velocity 3.3 times higher than the weakest (Sepharose CL-4B), whilst the DMA technique showed Capto Q to have a slurry deformation rate 8.3 times lower than Sepharose CL-4B. Capto Q is designed for capture and intermediate purification of proteins from large feed volumes. Results show a strong correlation between the two techniques. This technique was conducted on seven resins with varying degrees of mechanical robustness and the results were compared to pressure-flow test results on the same resins. To address these issues, we have developed a dynamic mechanical analysis (DMA) technique that characterises the mechanical properties of resins based on the viscoelasticity of a 1 ml sample of slurry. There are a number of drawbacks with this method, including the potential need for several re-packs to achieve the desired packing quality, the impact of wall effects on experimental set up and the quantities of chromatography media and buffers required. Pressure-flow profiles are most commonly used to characterise these properties. ne/divinyl benzene resin which has been substituted with quaternary amine groups to yield the strong anion exchanger, Mono Q, or with methyl sulphonate. Q Sepharose Fast Flow, 20 cm packed bed, with water at 20C. Resins and chemicals Q Sepharose Fast Flow, Q Sepharose XL and Capto Q resins were purchased from GE Healthcare (Saclay, France), Q Cellune resin was. Mechanical characterisation of agarose-based resins is an important factor in ensuring robust chromatographic performance in the manufacture of biopharmaceuticals. Running conditions: AxiChrom 1000 for Capto S, Chromaflow 1000 for. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |